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peimax  (Kyfora Bio)


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    Structured Review

    Kyfora Bio peimax
    Peimax, supplied by Kyfora Bio, used in various techniques. Bioz Stars score: 99/100, based on 5705 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peimax/PEI+MAX+Transfection+Reagent/pmc12830312-38-0-1
    Average 99 stars, based on 5705 article reviews
    peimax - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    shRNA:

    Article Title: Role of WIF-1 in regulating chondrocyte dedifferentiation and matrix production through WNT pathway.
    Article Snippet: .. Lentiviral particles encoding WIF1 cDNA or WIF1-targeting shRNA were generated by transient transfection of HEK 293T cells using PEIMax (Polysciences). ..

    Article Title: Role of WIF-1 in regulating chondrocyte dedifferentiation and matrix production through WNT pathway
    Article Snippet: .. Lentiviral particles encoding WIF1 cDNA or WIF1-targeting shRNA were generated by transient transfection of HEK 293T cells using PEIMax (Polysciences). ..

    Article Title: Targeting the splicing factor CWC22 induces mitotic slippage through repression of BubR1 expression and CDK1 activity in cancer cells
    Article Snippet: The oligonucleotides for CWC22 shRNA (shCWC22#2 [sense] 5′-CCGGCAACAGAGGACATACGAAATTCAAGAGATTTCGTATGTCCTCTGTTGTTTTTGCTAGC-3′ and [antisense] 5′-AATTGCTAGCAAAAACAACAGAGGACATACGAAATCTCTTGAATTTCGTATGTCCTCTGTTG-3′) were annealed and inserted into the Tet-pLKO-puro vector (Tet-pLKO-puro/shCWC22#2) (a gift from Dmitri Wiederschain [Addgene plasmid #21915; http://n2t.net/addgene:21915 ; RRID: Addgene_21915]) ( ). .. To establish the Dox-inducible cell lines of CWC22, BUB1B, and CWC22 shRNA, Lenti-X 293T cells were cotransfected with 0.8 μg of pCAG-HIVgp, 0.8 μg of pCMV-VSV-G-RSV-Rev, and 1.2 μg of pLIX_402 vector harboring either construct or Tet-pLKO-puro/shCWC22#2 using PEIMAX (Polysciences) in a 35-mm dish. ..

    Generated:

    Article Title: Role of WIF-1 in regulating chondrocyte dedifferentiation and matrix production through WNT pathway.
    Article Snippet: .. Lentiviral particles encoding WIF1 cDNA or WIF1-targeting shRNA were generated by transient transfection of HEK 293T cells using PEIMax (Polysciences). ..

    Article Title: Role of WIF-1 in regulating chondrocyte dedifferentiation and matrix production through WNT pathway
    Article Snippet: .. Lentiviral particles encoding WIF1 cDNA or WIF1-targeting shRNA were generated by transient transfection of HEK 293T cells using PEIMax (Polysciences). ..

    Article Title: CD164 is an endolysomal host factor for entry of Clade A New World Arenaviruses
    Article Snippet: .. Recombinant lentiviruses generated in this study (CD164 mutants, , S6C-D ) were produced by PEIMax (Kyfora Bio, 24765) mediated transfection of HEK293T cells with pCW62 expressing the gene of interest (CD164 mutant), pCAGGS-VSV-G, and psPAX2. ..

    Transfection:

    Article Title: Role of WIF-1 in regulating chondrocyte dedifferentiation and matrix production through WNT pathway.
    Article Snippet: .. Lentiviral particles encoding WIF1 cDNA or WIF1-targeting shRNA were generated by transient transfection of HEK 293T cells using PEIMax (Polysciences). ..

    Article Title: Drug-induced changes in connectivity to midbrain dopamine cells revealed by rabies monosynaptic tracing
    Article Snippet: Generation of AAVs from plasmids and AAV purification by Iodixanol gradient ultracentrifugation were carried out as described on Addgene’s website with minor modifications. .. Briefly, pHelper, pAAV2-5/pAAV2-8, and pJEP309/pJEP317-pAAV-U6-Cacna1eAC-EFS-GFP-KASH-pA plasmids were transfected into HEK293T cells using PEIMAX (Polysciences, cat# 24765-100). ..

    Article Title: Role of WIF-1 in regulating chondrocyte dedifferentiation and matrix production through WNT pathway
    Article Snippet: .. Lentiviral particles encoding WIF1 cDNA or WIF1-targeting shRNA were generated by transient transfection of HEK 293T cells using PEIMax (Polysciences). ..

    Article Title: Enhanced AAV production via rational design of a novel pHelper vector integrated with HSV-1 helper genes
    Article Snippet: .. PEImax (Polysciences, USA) was used as transfection reagent at a DNA: PEI mass ratio of 1:1.4. ..

    Article Title: CD164 is an endolysomal host factor for entry of Clade A New World Arenaviruses
    Article Snippet: .. Recombinant lentiviruses generated in this study (CD164 mutants, , S6C-D ) were produced by PEIMax (Kyfora Bio, 24765) mediated transfection of HEK293T cells with pCW62 expressing the gene of interest (CD164 mutant), pCAGGS-VSV-G, and psPAX2. ..

    Article Title: ATM Inhibition Enhances Knock-in Efficiency by Suppressing AAV-Induced Activation of Apoptotic Pathways.
    Article Snippet: .. HEK293T cells were transfected with a mixture of plasmid DNA and PEIMax (Polysciences) at a mass ratio of 1:6. ..

    Produced:

    Article Title: PTBP1 supports mouse spermatogenesis by facilitating cytoskeletal organization through the mTORC2-PKCα pathway in Sertoli cells.
    Article Snippet: The DNA fragment encoding the H1tetO promoter and shPtbp1 was subsequently subcloned into CS-RfA-ETR (e.g., CS-H1tetO-shRNA-ETR) using Gateway LR clonase (Thermo Fisher Scientific). pENTR4-H1tetOx1 without the shRNA sequence was used as a mock control (CSH1tetO-Mock-ETR). .. Lentiviral vectors were produced in 293T cells using PEImax (Polysciences, Warrington, PA, USA). ..

    Article Title: CD164 is an endolysomal host factor for entry of Clade A New World Arenaviruses
    Article Snippet: .. Recombinant lentiviruses generated in this study (CD164 mutants, , S6C-D ) were produced by PEIMax (Kyfora Bio, 24765) mediated transfection of HEK293T cells with pCW62 expressing the gene of interest (CD164 mutant), pCAGGS-VSV-G, and psPAX2. ..

    Recombinant:

    Article Title: CD164 is an endolysomal host factor for entry of Clade A New World Arenaviruses
    Article Snippet: .. Recombinant lentiviruses generated in this study (CD164 mutants, , S6C-D ) were produced by PEIMax (Kyfora Bio, 24765) mediated transfection of HEK293T cells with pCW62 expressing the gene of interest (CD164 mutant), pCAGGS-VSV-G, and psPAX2. ..

    Expressing:

    Article Title: CD164 is an endolysomal host factor for entry of Clade A New World Arenaviruses
    Article Snippet: .. Recombinant lentiviruses generated in this study (CD164 mutants, , S6C-D ) were produced by PEIMax (Kyfora Bio, 24765) mediated transfection of HEK293T cells with pCW62 expressing the gene of interest (CD164 mutant), pCAGGS-VSV-G, and psPAX2. ..

    Mutagenesis:

    Article Title: CD164 is an endolysomal host factor for entry of Clade A New World Arenaviruses
    Article Snippet: .. Recombinant lentiviruses generated in this study (CD164 mutants, , S6C-D ) were produced by PEIMax (Kyfora Bio, 24765) mediated transfection of HEK293T cells with pCW62 expressing the gene of interest (CD164 mutant), pCAGGS-VSV-G, and psPAX2. ..

    Plasmid Preparation:

    Article Title: ATM Inhibition Enhances Knock-in Efficiency by Suppressing AAV-Induced Activation of Apoptotic Pathways.
    Article Snippet: .. HEK293T cells were transfected with a mixture of plasmid DNA and PEIMax (Polysciences) at a mass ratio of 1:6. ..

    Construct:

    Article Title: Targeting the splicing factor CWC22 induces mitotic slippage through repression of BubR1 expression and CDK1 activity in cancer cells
    Article Snippet: The oligonucleotides for CWC22 shRNA (shCWC22#2 [sense] 5′-CCGGCAACAGAGGACATACGAAATTCAAGAGATTTCGTATGTCCTCTGTTGTTTTTGCTAGC-3′ and [antisense] 5′-AATTGCTAGCAAAAACAACAGAGGACATACGAAATCTCTTGAATTTCGTATGTCCTCTGTTG-3′) were annealed and inserted into the Tet-pLKO-puro vector (Tet-pLKO-puro/shCWC22#2) (a gift from Dmitri Wiederschain [Addgene plasmid #21915; http://n2t.net/addgene:21915 ; RRID: Addgene_21915]) ( ). .. To establish the Dox-inducible cell lines of CWC22, BUB1B, and CWC22 shRNA, Lenti-X 293T cells were cotransfected with 0.8 μg of pCAG-HIVgp, 0.8 μg of pCMV-VSV-G-RSV-Rev, and 1.2 μg of pLIX_402 vector harboring either construct or Tet-pLKO-puro/shCWC22#2 using PEIMAX (Polysciences) in a 35-mm dish. ..



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